Image preprocessing
The automated processing an analysis algorithms included in J-AST assume that images follow specific rules:
Dark background, bright colonies, high contrast
A single plate per image, single strip/disk per plate (learn more about handling such images)
No perspective, image captured from top
RGB colored images will be automatically converted to greyscale during processing steps.
Processing inverted images
If your image shows dark colonies on a bright background, you can preprocess them within J-AST by first selecting the images and then navigating to
Processing low-contrast images
If your images are low-contrast and automated algorithms tend to be not successful, try selecting the images and running .
02 December 2025