Multiple plates/disks/strips
J-AST is designed to handle only one plate per image, as well as only one DDA disk/E-Test strip per plate. Yet, you can still adapt J-AST to handle your images by uploading images multiple times and controlling the plate region.
Preparation
Upload your image multiple times (per plate, per disk/strip)
Apply all necessary metadata steps as with any other image
Multiple plates
If multiple plates are present, you will have to rely on annotating the plate region manually using the interactive editor included within J-AST. After configuring the plate area, run the manual pixel size calibration through , which will the correct pixel size information for the image(s).
Multiple disks/strips
Either find the plate automatically and run the associated pixel size calibration (automatically done by default in case of automated detection) or annotate the full plate manually (see above), ensuring that the pixel size calibration results are correct.
Follow this by using the interactive editor to remove regions that are not associated to the intended strip/disk.
All following analysis steps will be constrained to your annotated plate area.