J-AST Help

Finding the E-Test ZOI shape

For most analysis algorithms to work E-Tests need to be annotated with the ZOI shape. J-AST provides automated and interactive methods to achieve this task.

Automated E-Test ZOI shape detection

You can find the E-Test strip detection algorithm by first selecting the image(s) and then navigating to PROCESS | E-Test | Auto-detect ETest ZOI shape (v1). The algorithm has no parameters.

Supported assay

Due to the complexity of this problem, our algorithm only works decently if the contrast between the colonies and the ZOI is very high.

Comparison of algorithm inputs

Manual E-Test ZOI shape detection

If the algorithms do not produce the expected results or if you prefer to do a manual annotation, you can use our interactive tool. To open it, select the image and click the Edit button for the ZOI shape annotation on the right-hand side.

ZOI shape editor

To lean more about the interactive editor, follow our guide.

02 December 2025